Characterization of the RNAi efficiency in isolated alleles by silencing mom-2 essential gene. Each bar represents the percentage of unhatched eggs over the entire progeny laid. Error bars represent standard error of mean. The total progeny scored is indicated (n). Only a186, a190, a200, a202 alleles present a severe impairment in the RNAi machinery, below 50% of lethality. All animals, except the wild-type (wt) and rde-1(ne219), carry the transgenes gbIs4[Punc-25::smn-1(RNAi sas)]; oxIs12[Punc-47::GFP].