Fig. 1. Hypoxia alters Th17-cell response to AhR ligation in Crohn’s disease.

(A) Representative Western Blot analysis showing Th17-cell expression of AhR and ARNT under normoxic and hypoxic (2% O₂) conditions in one representative healthy subject (HS) and one patient with Crohn’s disease. Mean ± SEM AhR and ARNT density in Th17-cells (HS, n=6; Crohn’s patients, n=6). (B) Representative Western Blot analysis showing HIF-1α expression by Th17-cells of one HS and one Crohn’s patient. Mean ± SEM HIF-1α density in Th17-cells from the same HS and Crohn’s patients. Mean ± SEM of relative ENTPD1 (C), FOXP3 (D) and IL10 (E) mRNA expression by Th17-cells from HS (n=13) and Crohn’s patients (n=9).

Comparisons were made using one-way ANOVA or Kruskal-Wallis test, followed by Tukey’s or Dunn’s multiple comparison tests. *P≤0.05;**P≤0.01.