Skip to main content
. Author manuscript; available in PMC: 2019 Nov 1.
Published in final edited form as: J Autoimmun. 2018 Aug 8;94:122–133. doi: 10.1016/j.jaut.2018.07.022

Fig. 4. Hypoxia boosts the expression of AhR/ARNT-regulated ABC transporters.

Fig. 4.

Mean ± SEM (A) CYP1A1, (B) ABCB1 and (C) ABCC4 mRNA expression by Th17-cells from HS (n=10 for A; n=13 for B and n=9 for C) and patients with Crohn’s disease (n=6 for A; n=9 for B and n=7 for C). (D) Chromatin immunoprecipitation analysis showing enrichment of ABCB1 and ABCC4 mRNA expression in the promoter region bound by HIF-1α antibody, as compared to IgG negative control. Mean ± SEM ABCB1 and ABCC4 mRNA levels from three independent experiments performed using Th17-cells obtained from one healthy blood donor. (E) Mean ± SEM MDR1 and MRP4 activity, measured as percentage increase upon UCB stimulation in HS and Crohn’s disease patients (HS, n=10 for MDR1 and n=6 for MRP4; Crohn’s patients: n=8 for MDR1 and n=6 for MRP4). Comparisons were made using Wilcoxon signed-rank test (A-B) and paired t test (C, E). *P≤0.05;**P≤0.01.