Fig. 7. Immunological effects of combined treatment with UCB and MDR1/MRP4 inhibitors in DSS colitis.

WT mice (n=24) were treated with 3% DSS for 6 days and sacrificed on day 7. In additional n=24 mice, DSS treatment was replaced with normal water for additional 4 days. For the whole duration of experiment, mice were administered vehicle (n=12), UCB (n=12), RTV (n=12), or UCB plus RTV (n=12). Mean ± SEM frequency of (A) CD4⁺IL17⁺, (B) CD4⁺IL10⁺, (C) CD4⁺CD39⁺ and (D) CD4⁺FOXP3⁺cells among spleen, MLN, IEL, LP-derived mononuclear cells obtained from mice on day 7 or 10.

Comparisons were made using one-way ANOVA or Kruskal-Wallis test, followed by Tukey’s or Dunn’s multiple comparison tests. *P≤0.05;**P≤0.01;***P≤0.001.