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. Author manuscript; available in PMC: 2019 Nov 1.
Published in final edited form as: Pain. 2018 Nov;159(11):2306–2317. doi: 10.1097/j.pain.0000000000001337

Figure 1. Peripheral CGRP induces spontaneous grimace in free-moving mice.

Figure 1

(A) Experimental design. (B) Mean grimace scores of CD1 mice measured at baseline (B) and after receiving vehicle (PBS, 10 ml/kg IP) or CGRP (0.1 mg/kg IP). (C) Representative pictures at baseline and post vehicle or CGRP injection as indicated. Average between 3 experiments. First experiment assessed baseline, 5, 10 and 15 minute time-points (n=10 per group). Second experiment assessed baseline, 15, 30, and 60 minute time-points (n=10 per group). Third experiment assessed baseline, 30, 90 and 120 minute time-points (n=10). Therefore n=10 to 30 per group in this figure. Experiments were scored by two blinded individuals, error bars indicate ± SEM. Two-way (with factors treatment and time) ANOVA P < 0.0001 for treatment factor, Sidak’s multiple comparison test, ***P < 0.001, ****P < 0.0001 when comparing CGRP to vehicle group at corresponding time point.