Scored images obtained during the restrained MGS experiment (Fig. 3C, D, E, F) were used to measure eyelid fissure.
(A) CD1 in dark, (B) CD1 in light, (C) C57BL/6J in dark and (D) C57BL/6J in
light. Average of 2 experiments per strain, scored by 1 blinded individual
(n=10 per group for CD1 and n=20 per group for C57BL/6J,
± SEM). Two-way repeated measures ANOVA followed by Sidak’s
multiple comparison test to compare baseline and treatment conditions,
****P < 0.001. To
compare the effect of treatments, the deltas between the scores after treatment
from baseline were compared with an unpaired t-test,
ΔΔΔP < 0.001,
ΔΔΔΔP < 0.0001.