(A) C57BL/6J in dark, (B) C57BL/6J in light. Scored images obtained during the
restrained MGS experiments (Fig. 4A, B)
were used to measure eyelid fissure. Average of 2 experiments scored by 1
blinded individual (n=10 per group, ± SEM). Two-way repeated
measures ANOVA followed by Sidak’s multiple comparison test to compare
baseline and treatment conditions, *P < 0.05,
***P < 0.001,
****P < 0.001, not
significant (ns). To compare the effect of treatments, the deltas between the
scores after treatment from baseline were compared with a one-way ANOVA followed
by Dunnett’s multiple comparison test, ΔP <
0.05.