Table 1. Whole genome amplification and qPCR assay summary statistics.
Schistosome spp. | Location | Country | Collection date | No. schistosome positive WGA/Total no. WGA performed | % schistosome positive samples by qPCR | No. of patients with schistosome positive WGA/no. of patients | Average no. of spots tested per patient* | Average (+ sd) DNA conc. after WGA (ng.μL−1)** |
---|---|---|---|---|---|---|---|---|
S. mansoni | E. Africa | Tanzania | 2012 | 60/101 | 59.40% | 60/64 | 1.68 | 34 ± 5.19 |
W. Africa | Senegal | 2007–8 | 24/48 | 50% | 23/27 | 2.09 | 28.74 ± 5.08 | |
Niger | 2006–7 | 13/28 | 46.42% | 13/17 | 2.23 | 24.63 ± 5.04 | ||
Cameroon | 2007 | 0/14 | 0% | 0/6 | - | - | ||
S. haematobium | E. Africa | Zanzibar | 2011 | 65/138 | 47.10% | 62/80 | 2.22 | 22.45 ± 4.65 |
W. Africa | Niger | 2013 | 55/83 | 66.26% | 52/57 | 1.60 | 21.21± 2.85 |
We initially conducted WGA from a single miracidia per patient: further WGA were done only if the initial sample was schistosome negative by qPCR
Only shown for schistosome positive samples