TABLE 2.

RETROGRADE TRACER INJECTIONS^†,^‡

Strain§	Mouse ID	Tracer	Injection	Survival (d)	Immunohistochemistry
GAD67-EGFP+	ISM3	FG	~1uA/15 min	7	FG
GAD67-EGFP+	ISM4†	FG	2–8Ua/15 min	9	FG/PHOX2b
					FG/TH
GAD67-EGFP+	ISM5	FG	1–8uA/15min	10	FG/TH
GAD67-EGFP+	ISM8	FG	1–8uA/15min	13	FG/TH
GAD67-EGFP−	ISM10	FG	5uA/15 min	8	FG/TH
					FG/PHOX2b
GAD67-EGFP−	ISM13	FG	Not noted4	10	FG/TH
					FG/PHOX2b
GAD67-EGFP+	ISM17	FG	5 min4	8	FG/TH
					FG/PHOX2b
GAD67-EGFP+	ISM18	FG	3–5uA/2 min	6	FG(r)/TH
GAD67-EGFP+	ISM19	FG	2.5 min4	5	FG/TH

^†denotes case used in Figure 9

^‡All 9 mice were adults. Weights ranged from 27–47 gr. In the 8 cases where gender was noted, 7 mice were males and 1 was a female. In a few instances, injection parameters were not noted but were within the range reported for the other cases

^§All mice were heterozygous offspring of C57BL6/J and GAD67-GFP knockin mice (RRID:IMSR_RBRC03674) (Tamamaki et al., 2003)

^¶Abbreviations: FG- Fluorogold, TH- tyrosine hydroxylase