Figure 7.

SP-D deficiency increases NF-κB activation and renal inflammation in the kidney. Western blot analysis showed increased NF-κBp65 (A) and p-I-κB α (B) expression in the kidney of infected mice compared to sham mice, indicating increased inflammatory NF-κB signaling activation the in the kidney of infected mice. Quantitative analysis indicated that the levels of NF-κBp65 and p-I-κB α in the kidney of infected mice significantly differ with an order (KO > hTG > WT), suggesting that lack of pulmonary and/or renal SP-D increases the level NF-κB activation in the kidney of bacterial pneumonia. *p < 0.05, **p < 0.01, One-way ANOVA, Newman-Keuls multiple comparison post hoc test (n = 3/group). Pro-inflammatory cytokines were determined by ELISA assay for IL-6 (C) and TNF-α (D) in the kidney of infected WT, KO and hTG mice. The results showed significantly elevated levels of IL-6 and TNF-α in all infected mice with an order (KO > hTG > WT) at 48 h after infection, suggesting inhibitory effects of SP-D in the renal inflammation of pneumonia-induced AKI. *p < 0.05, **p < 0.01, One-way ANOVA, Newman-Keuls multiple comparison post hoc test (n = 6/group). Sham = Sham infection, Pneu = Pneumonia.