Figure 1.

Expression of reprogramming transcription factors in pancreatic organoids infected with multiple vectors. (A) Schematic of the doxycycline inducible multicolor vector system. Immunostaining showing co-expression of GFP and Ngn3 (B); LSS-Orange and Pdx1 (C); Tomato and MafA (D). Nuclei are counterstained with DAPI (B,D) or DRAQ7 (C). Scale bar: B, C, D: 20 μm. (E) Experimental schematic. Pancreatic organoids were infected with viruses encoding GFP-Ngn3, LSS-Orange-Pdx1 and Tomato-MafA and expression induced with doxycycline for 8 days. Genome-wide RNA sequencing was performed on organoid cells sorted for the different fluorescent marker combinations.