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. 2018 Oct 18;8:15374. doi: 10.1038/s41598-018-33838-5

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Ngn3 protein phosphorylation and endocrine reprogramming. (A) Western blot showing expression and phosphorylation of HA-tagged Ngn3 in pancreatic organoids. Tubulin is used as loading control. Full length western blots are provided in Supplementary Fig. S1A and S1B. (B,C) Expression of pools of 26 α-cell-enriched genes (B) and 151 β-cell enriched genes (C) in pancreatic organoids infected with multiple coloured vectors expressing combinations of Ngn3, Pdx1 and MafA, as labelled (graph bars represent pools of 26 α-cell genes and 151 β-cell genes, described in Dataset_S02 and Dataset_S03 of Supporting Information in Xin et al., PNAS 2016²⁹). Data represent average log2 fold change and error bars represent 95% confidence intervals of the mean.