Figure 5.

(A) The human ALPK1 protein expression level was measured 48 h after the start of transfection. Transfection of FreeStyle 293-F cells or FreeStyle CHO-S cells in 1 L Erlenmeyer cell culture flasks. The cells at a density of  6 × 10⁷ cells/mL in 500 mL FreeStyle 293-F expression medium and FreeStyle CHO expression medium, respectively, were transfected with pHH-GM1-ALPK1 or pCMV-8 X-His at a DNA:FreeStyle MAX Reagent ratio of 1:2.5 with a final DNA concentration of 5 μg/mL in the culture medium. At 6 h post-transfection, the culture was diluted with one volume of culture medium. (B) The ALPK1 protein production at different time points. (C) The cell growth rate: 1 × 10⁶ cells/mL FreeStyle 293-F cells were transfected with 2 μg/mL of plasmid DNA (pHH-GM1, pEGFP-C2, pFN21K-Halo and pcDNA3.1) and examined at various times (3, 5, 7 and 11 days).