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Kinetic parameters determined for purified GH43 enzymes with 6-chloro-4-methylumbelliferyl β-D-xyloside
| Enzyme | KM(mM) | kcat(s-1) | kcat/KM (s-1 mM-1) |
|---|---|---|---|
| 12_J03-18 | 0.48 ± .06 | 0.22 ± .02 | 0.45 ± .09 |
| 12_H03-13 WT | 0.19 ± .03 | 0.80 ± .06 | 4.2 ± 0 .7 |
| 12_H03-13_E507A | 0.14 ± .01 | 0.73 ± .02 | 5.2 ± 0.4 |
