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. 2018 Oct 18;8:15430. doi: 10.1038/s41598-018-32024-x

Figure 2.

Figure 2

Identification of the expression of the BLG in the milk. (A) Characterization of the BLG-free milk by SDS-PAGE. M, protein marker; BLG, 2.5 µg of commercial BLG (Sigma) as a positive control; line 3–5, 1 µl of wild-type cow raw milk from different days; line 6–8,1 µl of #111027 (BLG-free) raw milk from different days. (B) Characterization of the BLG-free milk by Western blot. BLG, 2.5 µg of commercial BLG (Sigma) as a positive control; line 2–4, 1 µl of wild-type cow raw milk from different days; line 5-7, 1 µl of #111027 (BLG-free) cow raw milk from different days. (C) Concentration of the main proteins in the control milk and BLG-free milk. Results are shown as means ± SD. *P < 0.05, significantly different from control milk.