Figure 2.

CT and TT carriers, but not CC individuals, have a significantly greater proportion of naïve CD8 T-cells with a SIRPγ^low phenotype. Flow cytometry staining for CD27 and CD45RO was used to analyze SIRPγ^low CD8 T-cells in the naïve (CD27+ CD45RO−), central memory (CD27+ CD45RO+), effector memory (CD27−CD45RO+) and terminally differentiated CD27−CD45RO−) fractions. Detailed gating strategy is shown in Supplementary Fig. 1. In CC individuals, SIRPγ^low CD8 T-cells were essentially absent from the naïve pool, whereas in CT and TT carriers, SIRPγ^low CD8 T-cells were present in the naïve pool at increasing frequencies (A). Representative dot plots are shown in (B), where the blue population indicates SIRPγ^low CD8 T-cells and the red population corresponds to the SIRPγ^high CD8 T-cells. One-way ANOVA with Tukey’s posthoc test was performed and p < 0.05 was considered significant.