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. 2018 Oct 18;8:15440. doi: 10.1038/s41598-018-33901-1

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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CD8 T-cells from TT carriers display the same phenotypic and functional profile as SIRPγ^low CD8 T-cells from CC and CT carriers. (A) CD8 T-cells from CC, CT and TT carriers were evaluated for their expression of the indicated molecules by flow cytometry. Intracellular staining was performed for T-bet. In the case of CC and CT, cells were gated for SIRPγ-high vs. low cells. (B) PBMC samples from CC, CT, TT carriers were stimulated with PMA/Ionomycin/brefeldin A for five hrs before intracellular staining for IFN-γ and TNF-α. CD8 T-cells from CC and CT carriers were gated on SIRPγ^low vs. high cells and evaluated for frequency of cells producing the indicted cytokines. 1-way ANOVA with with Tukey’s posthoc test was performed and p < 0.05 was considered significant.