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. 2018 Oct 18;8:15440. doi: 10.1038/s41598-018-33901-1

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Expression of effector vs. memory-associated transcription factors in SIRPγ^low versus SIRPγ^high CD8 T-cells. Transcription factor analysis was performed on RNA sequencing data presented in Fig. 3. For RNA sequencing, SIRPγ^low and SIRPγ^high CD8 T-cells were FACS sorted from two CT carriers and subjected to RNA sequencing. Heat maps of SIRPγ^low versus SIRPγ^high CD8 T-cells are shown (A). SIRPγ^low CD8 T-cells have significant downregulation of transcription factors associated with functional memory formation and are enriched in transcription factors upregulated in effector CD8 T-cells (A). The transcription factor profile was confirmed in CD8 T-cells from three TT carriers vs. CC carriers by quantitative polymerase chain reaction (qPCR) (B). The y-axis shows delta-delta Ct values, after transformation to a logarithmic scale for LEF1, TCF7, EOMES, and PRDM1 in CC vs. TT CD8 T-cells. T-test was performed and p < 0.05 was considered significant.