Fig. 2. Antiproliferative activities of YLT-11 against human breast cancer cell lines.

a Four different breast cancer cells were treated with increasing doses of YLT-11 for 24, 48, 72, and 96 h, respectively. Each point represents the mean ± SD for three independent experiments (*p < 0.05, **p < 0.01, ***p < 0.001 vs. vehicle control).  b Effects of YLT-11 on cell colony formation after treatement for 2 weeks. Quantification is shown in the right panel. Columns, means (n = 3); bars, standard deviation (*p < 0.05, **p < 0.01, ***p < 0.001). c YLT-11 inhibited MDA-MB-468 and MDA-MB-231 cell proliferation. The EdU incorporation assay was examined on cells after exposure to YLT-11 for 24 h. EdU-positive (marked by red fluorescent staining) and Hoechst 33342 staining (marked by blue fluorescent staining) cells represented the proliferating and total cells, respectively. Images shown are representatives of three independent experiments. Scale bars, ×20 for micrograph