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. 2018 Oct 18;9(11):1066. doi: 10.1038/s41419-018-1071-2

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© The Author(s) 2018

Open Access This article is licensed under a Cree Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Cell morphological alterations and nuclear changes of MDA-MB-468 and MDA-MB-231 cells were analyzed by staining with Hoechst 33342 (10 mg/mL) and visualized using a microscope after treatment with increasing doses of YLT-11 for 24 h. b MDA-MB-468 and MDA-MB-231 cells were treated with YLT-11 for 48 h and were detected by flow cytometry after Annexin V/PI staining. c The expressions of cleaved caspase-3 and cleaved PARP1 were determined via western blotting. Protein expressions were quantized by densitometry analysis using ImageJ (shown in the right panel). Data are expressed as mean ± SD for three independent experiments. Columns, mean; bars, SD (*p < 0.05, **p < 0.01, ***p < 0.001)