Table 1.

Insemination Treatment Groups.

Treatment	Epididymal Flush (500 µL)	Seminal Plasma (250 µL)
1	Infected Male	Infected Male
2	Infected Male	Sham-infected Male
3	Sham-infected Male	Infected Male
4	Sham-infected Male/Spike	Sham-infected Male/Spike
5	104 CCID50/100 μL ZIKV PRVABC59 in MEM
6	Sham-infected Male	Sham-infected Male

Components of each treatment group. Each epididymal flush totaled 500 µl of fluid and seminal plasma totaled 250 µl of fluid. The epididymal flush and seminal plasma fluid were combined and coincubated creating the artificial insemination inoculum.; CCID₅₀ = 50% cell culture infectious doses; MEM = Minimal Essential Media.