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. 2018 Oct 18;8:15383. doi: 10.1038/s41598-018-33784-2

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Inhibition of XPO1 by selinexor regulates the expression of anti- and pro-apoptotic Bcl-2 family member proteins. (A) LN229, T98G and U87 GBM cells were treated with ABT263, selinexor or the combination. Alternatively, cells were treated with increasing concentrations of selinexor for 24 h. Thereafter, whole cell protein lysates were collected and analyzed by conventional western blotting for the expression of Mcl-1, Bcl-2, Bcl-xL, Noxa and Actin. All concentrations are in μM. (B) LN229 GBM cells were treated with selinexor or the combination treatment of ABT263+ selinexor for the indicated time points (time course experiment). Thereafter, whole cell protein lysates were prepared and analyzed for the expression of Mcl-1. Hrs: hours. (C) LN229 GBM cells were treated with vehicle, ABT263, selinexor or the combination treatment. After 6 h, RNA was harvested, reverse transcribed and subjected to real-time PCR analysis for Mcl-1.