Fig. 3.

DNA-PK inhibition increases the replication of M1 virus in refractory tumour cells. a HCT-116 or BxPC-3 cells were infected with M1-GFP (MOI = 1) in the presence or absence of NU7441 (1 μM) for 24 h, after which the phase-contrast and fluorescence microscopy images were captured. Scale bar: 50 μm. b HCT-116 or BxPC-3 cells were infected with M1-GFP (MOI = 1) in the presence or absence of NU7441 (1 μM). Single-step growth analyses were conducted. n = 4. c HCT-116 or BxPC-3 cells infected with M1-GFP (MOI = 1) in the presence or absence of NU7441 (1 μM), viral structural protein E1 and the non-structural protein NS3 were analyzed by western blot after treatment. d HCT-116 or BxPC-3 cells were treated with siRNA targeting DNA-PKcs (48 h), followed by M1 virus infection (MOI = 1), and then processed for qRT-PCR to measure the viral gene E2 and NS1. n = 3. Data represent the mean ± SD in b, d. *P < 0.05; **P < 0.01; ***P < 0.001 by two-way ANOVA for b or one-way ANOVA for d