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. 2018 Oct 18;8:15385. doi: 10.1038/s41598-018-33979-7

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© The Author(s) 2018, corrected publication 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The HSC niche of CKD-MBD mice is functionally impaired and affects the repopulating capacity of HSC. (A) BM cells obtained from untreated donors were harvested and transplanted into lethally irradiated, congenic CKD-MBD or sham-operated recipients. At 18 weeks after transplantation, BMC obtained from primary recipients were harvested, pooled, analyzed for LSK chimerism (E) and re-transplanted into lethally irradiated secondary recipients. At three-week intervals, donor chimerism was analyzed in (B–D) primary and (E–G) secondary recipients. Donor-derived (B,E) CD45⁺ cells and (C,F) granulocytes were assessed. (D,G) At 18 weeks after transplantation the percentage of donor-derived CD45⁺ cells, granulocytes, Lin^negSca-1^posc-kit^hi (LSK) cells and CD34^negLSK (HSC) in the spleen and BM were analyzed. ^*p < 0.05 and ^**p < 0.01. In panel B–G, each dot represents a single mouse.