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. 2018 Oct 18;8:15345. doi: 10.1038/s41598-018-33738-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(a) Schematic of the microfluidic device, which contains a whole blood sample inlet and a buffer inlet, respectively. After the whole blood sample flowed to the bifurcation region (orange zone), plasma and RBCs were extracted to plasma zone and RBC zone, respectively. WBCs flowed to the main channel and were trapped in the WBC zone. (b) Photograph of the plasma zone and RBC zone entrance. Green particles are packed beads, and red particles are RBCs. (c) Photograph of the WBC zone with trapped WBCs.