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. 2018 Oct 18;8:15345. doi: 10.1038/s41598-018-33738-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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WBC seeding pattern in the trapping units of the microfluidic device. (a) Schematic and time-lapse fluorescence images of WBC zone. (b) Trapped WBC numbers in top, middle, and bottom regions at different time points. The red dashed lines are the regression lines of each region, representing the WBC capture rate. (c) Enlarged image of the yellow box showing the WBC seeding profile.