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. 2018 Oct 18;8:15345. doi: 10.1038/s41598-018-33738-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Simultaneous extraction of plasma, RBCs and on-chip white blood cell trapping. (a) Image of the microfluidic device showing the position of each zone. (b) Standard curve of the absorbance intensity versus dilution factor of manually diluted blood (black rectangles and red dashed line). The blue star represents the absorbance and corresponding dilution factor (0.67x) of extracted plasma in this test. (c) Blood type test images from the extracted RBC solution. The red triangles represent blood agglutination. (d) WBC seeding pattern in the WBC zone.