Figure 4.

After 7 days of spheroid culture, TMZ (600 µM, right inlet) and BEV (7.5 µM, left inlet) were applied to the brain cancer chip. Effects of the drug treatment on the cancer spheroids were visualized by the shrinking spheroid volume and the disaggregation of dead cells from the spheroids in patient 1 (a), patient 2 (b), and patient 3 (c). Seven days after drug administration, the cells were briefly rinsed with PBS and loaded with 0.4% trypan blue for semi-quantitative cell viability (d). Quantitative analysis was performed off chip using a trypan blue exclusion. Scale bar is 100 µm.