Fig. 1. AZD8055 and rapamycin inhibited mTOR signaling pathway followed by inducing autophagy.

a, b U87 and U251 GICs were treated with AZD8055 and rapamycin for 24, 48, 72, and cell viability was detected using CCK-8 assay. c–e U87 and U251 GICs were treated with various concentrations of AZD8055 for 48 h and rapamycin for 24 h. The cells were harvested, and the effects of AZD8055 and rapamycin on the protein expression of the mTOR pathway and autophagy-related were detected by western blot. Data are shown as means ± s.d., n = 3, ^#P = NS, *P < 0.05, **P < 0.01, Student’s t-test. f Immunofluorescence staining of U87 and U251 GICs, which treated by DMSO, AZD8055 (0.3 μM) and rapamycin (3 μM). The nuclei were stained with DAPI and the antibody against LC3B and p62. Images were captured by laser confocal microscope ( × 400), scale bar = 100 μm