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. 2018 Oct 18;8:15402. doi: 10.1038/s41598-018-33865-2

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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HPV16-immortalized cells from TZ and endocervix express more phospho-Akt. (a) HPV16-immortalized cell lines from ectocervix, TZ, or endocervix were maintained for 10 days on collagen rafts with cervical stromal cells and then stained with fluorescent antibody to phospho-Akt. Control indicates cells that were stained with an irrelevant antibody. Dotted lines indicate the position of the basement membrane and the upper extent of epithelial cells. (b) Mean percentage of phospho-Akt positive cells ± standard error of nine different HPV16-immortalized cell lines (3 from ectocervix, TZ and endocervix). Bars show values that are statistically different (3 asterisks = p < 0.001).