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Two-photon fluorescence imaging of probe NIR-SN-GGT in A2780 cells. (a) bright imaging; (b) one-photon imaging; (c) merged imaging from (a,b); (d) two-photon imaging; (e) merged imaging between (a) and (d); (f) fluorescence emission intensities of NIR channel were measured as averages of 9 regions of interest (ROIs) from different treated A2780 cells. Error bar = RSD (n = 9). (OP mode) λex = 488 nm and λem = 655-755 nm; (TP mode) λex = 800 nm and λem = 575–630 nm. Scale bar = 20 μm.
