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. 2018 Oct 12;9:2370. doi: 10.3389/fimmu.2018.02370

Figure 7.

Figure 7

Immunofluorescent staining of mouse spleen for germinal center B cells. Mice received intraperitoneal VCI (IMQ 50 μg + VP 10 μg), IMQ (50 μg), VP (10 μg), or PBS for 3 days, and then were challenged with 10 × LD50 of H1N1/415742Md virus intranasally. At 4 days post virus challenge, spleens were taken and processed as frozen tissue sections. Consecutive sections were stained with anti-mouse CD45R/B220 and anti-mouse Ki67 or anti-mouse CD45R/B220 and anti-mouse GL7 antibodies. The stained tissues were examined under fluorescent microscope Olympus BX53. B cell follicles expressing B220/Ki67, B220/GL7 were counted and calculated. (A) Representative images of spleens stained with CD45R/B220 (green), Ki67 (red), GL7 (red) and DAPI (blue). Original magnification 100 × and 200 × . (B) Percentage of B220+/GL7+ B cell follicles in spleen at 4 days post virus challenge n = 3. †††p < 0.001 (compared with PBS group). ***p < 0.001; (comparing VCI group with IMQ group).