Figure 3.

Uptake of HPV(+) exosomes by immune cells. (A) Human monocyte-derived iDC were co-incubated with exosomes isolated from supernatants of SCC90 cell line [HPV(+)] and labeled with a PKH26 dye (red) as described in Methods. Confocal microscopy was performed at the indicated time points. (B) Human primary T cells were activated as described in Methods and co-incubated with SCC90 cell line-derived exosomes for different time periods. (A,B) Cells were acid washed to remove surface-bound exosomes. Nuclei are blue, exosomes are red and F-actin is green. Representative images are from 1/3 experiments performed with the recipient cells obtained from different donors. In an experiment performed with HPV(−) exosomes, uptake of labeled vesicles into iDC or T cells was not different from that in images shown for HPV(+) exosomes.