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. 2018 Oct 12;8:445. doi: 10.3389/fonc.2018.00445

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Copyright © 2018 Ludwig, Sharma, Theodoraki, Pietrowska, Yerneni, Lang, Ferrone and Whiteside.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Co-incubation of HPV(+) or HPV(−) exosomes with primary lymphocyte subpopulations. (A) Representative flow cytometry data for exosome-mediated inhibition of CD69-expression levels on the surface of human activated CD4⁺ T cells (top row). (B) Representative data for suppression of CFSE-labeled CD4⁺ T cell proliferation (bottom row). (C) The data for relative suppression of CD69 expression levels on T cells, and in (D) of T cell proliferation by exosomes isolated from HPV(+) and HPV(−) exosomes are compared. The data in (C,D) are means ± SD for 3 experiments performed with exosomes isolated from supernatants of each of the 5 HNC cell lines studied. nsd, no significant difference.