Table 2.
Experimentally validated targets (among BCa hub genes previously identified by WGCNA) of hub miRNAs recently identified in BCa (21).
| Hub miRNAs | Targeted hub genes | Validation assay | Cell lines | References |
|---|---|---|---|---|
| miR-1-1/2 | CENPF | Proteomics (pSILAC) | HeLa (human cervical cancer) | (71) |
| FBN1 | ||||
| HERC2 | ||||
| KIF2C | ||||
| KIF4A | ||||
| UBE2I | ||||
| miR-28 | PTPRJ | PAR-CLIP | HCT116 (human colon cancer) | (72) |
| miR-133a-1/2 | CDCA8 | PAR-CLIP | C8166 (HIV-1 infected human T cells) and TZM-bl (HIV-1 infected human epithelial cells) | (73) |
| FBN1 | Microarray | T24 and KK47 (human bladder cancer) | (58) | |
| miR-139 | MMP11 | Luciferase reporter assay, Microarray, qRT-PCR, Western blot | T24 and BOY (human bladder cancer) | (64) |
| miR-143 | BRD2 | PAR-CLIP | HCT116 (human colon cancer) | (72) |
| CDC25B | ||||
| COL3A1 | In situ hybridization, qRT-PCR, Western blot | NF-38 (human normal gastric fibroblasts) and CaF-38 (human cancer-associated fibroblasts) | (74) | |
| COL5A2 | Microarray | USSCs (human unrestricted somatic stem cells) | (75) | |
| miR-195 | CEP55 | PAR-CLIP | HEK293S (human embryonic kidney) and MCF7 (breast cancer) | (76–79) |
| PTPRJ | HITS-CLIP | Jijoye (EBV-transformed B cells) | (80) | |
| PURA | ||||
| TRAK1 | HITS-CLIP | HEK293S (human embryonic kidney) and fresh-frozen human heart tissues | (81, 82) | |
| UBE2I | Microarray, qRT-PCR | PBMCs (peripheral blood mononuclear blood cells) | (83) | |
| miR-6507 | CDC25B | PAR-CLIP | HCT116 (human colon cancer) | (72) |
| CDCA8 | ||||
| DCAF8 | PAR-CLIP | HEK293S (human embryonic kidney) | (76) | |
| PRC1 | ||||
| CENPA | PAR-CLIP | hESCs (human embryonic stem cells) | (84) | |
| CEP55 | PAR-CLIP | BC-1 and BC-3 (KSHV-infected primary effusion lymphoma) | (85) |
pSILAC, pulsed Stable Isotope Labeling with Aminoacids in Cell culture; PAR-CLIP, PhotoActivatable Ribonucleoside-enhanced CrossLinking and ImmunoPrecipitation; HITS-CLIP, HIgh-Throughput Sequencing of RNA isolated by CrossLinking and ImmunoPrecipitation.