Figure 2.

Assigned 2D [¹⁵N,¹H] HSQC spectra for gHH_44 collected in the oxidized (a) and reduced state (b). Spectra collected in 20 mM sodium acetate, pH 4.8 buffer, 20°C, at 800 MHz ¹H resonance frequency without (a) and with (b) 2.5 mM TCEP. Amide side chain resonance pairs are connected by a red dashed line and are folded in the reduced spectrum. Note that the ¹⁵N chemical shift range shown is the same in both spectra, while the ¹⁵N carrier frequency is different.