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. 2018 Oct 12;9:2405. doi: 10.3389/fmicb.2018.02405

FIGURE 6.

FIGURE 6

Effect of phosphorylated OmpR on c-di-GMP concentration, PDE activity, and PDE-related genes expression. (A) c-di-GMP concentration of WT, ΔompR, and ompRD55A strains in LB broth with 400 mM NaCl (solid gray bars) or without (open white bars) was quantified by ELISA according to the manual (Wuhan EIAab Science). Relative percentage of c-di-GMP content was calculated by the c-di-GMP concentration of extracts is relative to that of WT strain. (B) The PDE activity of crude extracts of WT, ΔompR, and ompRD55A strains in LB broth with 400 mM NaCl (solid gray bars) or without (open white bars) was determined by using bis-pNPP as substrate and measuring the absorbance at OD410. Relative percentage of PDE activity was calculated by the OD410 of crude extracts is relative to that of WT strain. (C) qRT-PCR analyses of the PDE-related genes expression for WT and ompRD55A strains in LB broth with 400 mM NaCl. The results are representative of three independent experiments. Error bars indicate standard deviations. ∗∗P < 0.01 compared to the indicated group.