Table 1.
Bacterial strains and plasmids used in this study.
| Strains or plasmids | Descriptions | Reference or source |
|---|---|---|
| K. pneumoniae | ||
| CG43S3 | CG43 Smr | Lai et al., 2001 |
| ΔompR | CG43S3ΔompR | This study |
| ompRD55A | CG43S3ompRD55A | This study |
| ΔlacZ | CG43S3ΔlacZ | Lin et al., 2006 |
| ΔlacZ-ΔompR | CG43S3ΔlacZΔompR | This study |
| ΔlacZ- ompRD55A | CG43S3ΔlacZompRD55A | This study |
| E. coli | ||
| BL21(DE3) | F- ompT hsdSB[rB-mB-]gal dcm [DE3] | New England Biolabs |
| S17-1 λ pir | hsdR recA pro RP4-2 [Tc::Mu; Km::Tn7] [λpir] | Miller and Mekalanos, 1988 |
| Plasmids | ||
| yT&A | ApR, TA PCR cloning vector | Yeastern |
| pKAS46 | Apr, Kmr, Positive selection suicide vector, rpsL | Skorupski and Taylor, 1996 |
| pACYC184 | Cmr, Tcr, plasmid with p15A origin of replication | Chang and Cohen, 1978 |
| pmrkAZ15 | Cmr, 402 bp fragment containing the region upstream of mrkA cloned into placZ15 | Lin et al., 2016 |
| pompR | Cmr, 1079 bp fragment containing an ompR allele cloned into pACYC184 | This study |
| pET30b-OmpR | Kmr, 716 bp fragment encoding full-length ompR allele cloned into pET30b | This study |