Table 2.
Primers used in this study.
| Primer | Sequence (5′ → 3′) | Enzyme cleaved | |
|---|---|---|---|
| GT254 | GGGATCCAGACCGTCTGGGGTCT | BamHI | |
| GT255 | CTCTAGACAGCGGGTGCATACG | XbaI | |
| GT256 | CGGATCCATCTGTTCGGCGTTC | BamHI | |
| GT257 | CGAGCTCTACCTGTTCGGCTCTGGC | SacI | |
| GT284 | GGGATCCGCTCTCCATCAATGCTAA | BamHI | |
| GT285 | GAGATCTGAAAGACGTAGCGTACAGC | BglII | |
| GT288 | CGGATCCAGACAAAATGGAGGGAACCCTA | BamHI | |
| GT290 | GCAATAGCAACATTCTGATTGG | ||
| GT336 | CCATATGCAAGAGAATTATAAGATTCT | NdeI | |
| GT337 | GAAGCTTGCCTTAGAACCGTCCGGGAC | HindIII | |
| GT356 | ATGCTGCCGGGCGAAGATGGT | ||
| GT357 | TTCTAGATACGAATAAACAGCCA | XbaI | |
| GT363 | CAGAGCCAGCACCATCAGGTGGA | ||
| GT367 | GACATCAATCTGAAAGGCCAG | ||
| GT368 | AGGGATCACCACTGCCAGAA | ||
| CC323 | GGATCCTGCATGCTGTTGCGGTCAC | BamHI | |
| CC324 | GGATCCGCGGTTGCCATTGCTGCAGAG | BamHI | |
| SY01 | GGGATCCGGTAGTCCATCTCCTGCTTCA | BamHI | |
| SY02 | GAAGCTTGACGAACAGCAAGGTGACG | HindIII | |
| For qRT-PCR | Sequence (5′ → 3′) | TaqMan probes | Target |
| RT11 | GGTAGGGGAGCGTTCTGTAA | 67 | 23S rRNA |
| RT12 | TCAGCATTCGCACTTCTGAT | ||
| RT29 | TAAGCAAACTGGGCGTGAA | 20 | mrkA |
| RT30 | TAGCCCTGTTGTTTGCTGGT | ||
| GT46 | GTTTAAGTTCCGCCATCTCG | 120 | mrkH |
| GT47 | TTGCGCTTGGCTTCTAAGAT | ||
| GT42 | AGTTATGCCGATGTCATCCAT | 59 | mrkI |
| GT43 | GATTCTGATGGCAGAAATATCCTT | ||
| GT54 | TTTCGAGGTAACCGAAAACG | 84 | mrkJ |
| GT55 | GAGGTATCCTGTGGGCTCTG | ||
| RT159 | CTGGCTGGATGATTTTGGTC | 67 | D364_06025 |
| RT160 | CCACTTTGACGCAATCGAA | ||
| RT161 | CGTAGGCTGGCTGATGGA | 40 | D364_08130 |
| RT162 | TCTGTCTCGGTGACCTCGAT | ||
| RT164 | GGGTTTGCTGATATCGATGG | 67 | D364_13295 |
| RT165 | AGCCAACGTTTTGACATGCT | ||
| RT169 | ATCGATTTTCGCCATCAGAG | 70 | fimK |
| RT170 | GAAAAGCAGTCGTCCAGCAT | ||
| RT175 | AATGGTCATCCGGGAAGC | 20 | yjcC |
| RT176 | CCGCTGAACACAACTCACC | ||
| RT244 | GCATCGTAACCTGGTCTTCAA | 36 | D364_02175 |
| RT245 | GAGCAGGCTGGTGAAAATG | ||
| RT273 | CCAGGATCCTCGCCACTAT | 22 | D364_04060 |
| RT274 | TCCCGACCTTACCAAAACG | ||
| RT275 | CGCCGGTACTGATCCAAT | 28 | D364_11845 |
| RT276 | ATTCACCACCAGCATCACG | ||
| RT277 | GCTACCTGGTGCGCAATATAA | 68 | D364_16830 |
| RT278 | TCGCCATAAATACAGCGTAGC | ||