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. 2018 Sep 17;194(2):153–165. doi: 10.1111/cei.13195

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© 2018 The Authors. Clinical & Experimental Immunology published by John Wiley & Sons Ltd on behalf of British Society for Immunology

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Comparison of enzyme‐linked immunosorbent assay (ELISA) reaction of rabbit polyclonal antiserum with Bacteroides fragilis and human ubiquitin. (a) Anti‐recombinant human ubiquitin (Hubb) antiserum and (b) anti‐recombinant B. fragilis ubiquitin (rBfUbb) antiserum with rBfUbb (diamond), synthetic Bacteroides fragilis ubiquitin (sBfUbb; square) and Hubb (triangle). Black, immune serum; blue, preimmune serum. Initial dilution 1/500. Mean of two or more replicate experiments ± SD.