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. 2018 Sep 17;194(2):153–165. doi: 10.1111/cei.13195

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© 2018 The Authors. Clinical & Experimental Immunology published by John Wiley & Sons Ltd on behalf of British Society for Immunology

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Comparison of immunoglobulin (Ig)G reaction of human serum samples with sBfUbb and Hubb by enzyme‐linked immunosorbent assay (ELISA). (a) Plot of optical density (OD) readings for BfUbb versus Hubb (1/90 serum dilution). Category: autoimmune antibodies (ANA), diamond; rheumatoid arthritis (RA), open circle; multiple sclerosis (MS), x; ulcerative colitis (UC), triangle; coeliac, open square; allergy test negative (allergy–ve), solid circle; healthy volunteer (HV), solid square. (b) Plot of OD reading versus serum category (1/90 serum dilution) for reaction with sBfUbb (grey) and Hubb (black). Bar = mean OD reading. (C) Percentage within sample category (1/90 serum dilution) with an OD > 2 (solid fill) and OD > 2·5 (diagonal stripes); sBfUbb (grey), Hubb (black). *Indicates statistically significant difference with the healthy volunteer samples by Fisher’s exact test (two‐sided).