Figure 2.
Panobinostat disrupts anti‐inflammatory response to Candida albicans. (a,b) Buffy coat peripheral blood mononuclear cells (PBMCs) were isolated, cells were pretreated with panobinostat, etinostat and RGFP966 (1 h) and then incubated for 24 h with heat‐killed C. albicans conidia [1 × 106 colony‐forming units (CFU)/ml]. Supernatants were collected and stored at –20ᵒC until interleukin (IL)‐1Ra (a) and IL‐10 (b) induction was measured by enzyme‐linked immunosorbent assay (ELISA) [mean ± standard error of the mean (s.e.m.), n = 5 *P < 0·05, **P < 0·01, two‐way analysis of variance (anova) with Bonferroni post‐test].