Figure 6.

RGFP966 does not modulate adaptive cytokine immune responses to Candida albicans or Staphylococcus aureus in healthy control cells. (a–c) Peripheral blood mononuclear cells (PBMCs) were freshly isolated from healthy controls. Cells were incubated for 6 days in the presence or absence of C. albicans [1 × 10⁶ colony‐forming units (CFU)/ml] or heat‐killed S. aureus (1 × 10⁷ CFU/ml). At day 6, supernatants were removed and replaced with freshly prepared stimuli (C. albicans or S. aureus) in combination with or without RGFP966. Cells were maintained in these conditions for an additional 24 h before supernatants were collected and stored at –20ᵒC until (a) interferon (IFN)‐γ, (b) interleukin (IL)‐17 or (c) IL‐22 production was measured by enzyme‐linked immunosorbent assay (ELISA) (median, n = 3, no statistical analysis was performed).