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. 2018 Oct 12;10:34. doi: 10.3389/fnsyn.2018.00034

FIGURE 2.

FIGURE 2

Paired recordings reveal AMPAR rectification at PC-BC but not PC-MC synapses (A) 2-photon microscopy maximum intensity projection of a quadruple recording where a connection from morphologically identified cell 1 (PC) to cell 3 (BC) was measured. Scale bar: 50 μm. (B) AMPAR responses to PC 1 stimulation in BC 3 were recorded at various holding potentials (from –60 to + 60 mV). Inward rectification in the presence of intracellular spermine indicated the presence of CP-AMPARs. Scale bars: 10 ms, 100 pA. (C) In another paired recording, the AMPAR currents recorded from an MC in response to stimulation of the presynaptic PC did not show inward rectification, suggesting the absence of CP-AMPARs. Scale bars: 50 pA. (D) The cell-specific difference in rectification was robust, as shown by the pooled values of rectification index (RI) for PC-BC (red) and PC-MC (blue) paired recordings. Modified from (Lalanne et al., 2016) with appropriate permissions.