Fig. 5.

Effect of SA on the reactive oxygen species of anthers under control and heat stress. The anthers were incubated with 5 μM DCFH-DA, and were measured after 30 min by a fluorescence microscope. a, the fluorescence images of the anthers; b, these data were obtained from the fluorescence images (n = 10). Vertical bars denote standard deviations (n = 10). Different letters indicate significant differences between SA treatments under control and heat stress (P < 0.05)