Fig. 2.

Specific subcellular localization of Ac-MTs and PolyE-MTs in PPLL and platelets. a Ac- and polyE-MT staining of D723H cells at different times (1, 5, and 24 h) of spreading on fibrinogen. Bars 20 μm. High magnification images of the merge signals are shown. Bar 5 μm. Ac- and PolyE-MT staining do not completely overlap. b Western blot of D723H and WT expressing CHO cell lysates successively probed with antibodies against Ac-Tub, PolyE-Tub, total tubulin, and vinculin; molecular weight markers in kilodalton are indicated on the right. c Images of actin staining, Ac-MT, and PolyE-MT decoration of the marginal band MT ring in purified platelets spreading on glass coverslips show that marginal band MT acetylation and polyglutamylation content are not perfectly overlapping. Bar 2 μm. d High magnification images of actin staining and Ac- and PolyE- decoration of marginal band MTs in a purified round platelet spreading on glass coverslips (left panels) and high and low magnification of MT bundles in a D723H swelling (right panels). Bars 2 μm. Ac-MT/PolyE-MT staining merges are shown with two cross bars of respectively 2 and 4 μm