Figure 4.
Spatiotemporal control of PPG-idasanutlin. (a) Schematic representation of microwell setup for laser irradiation of individual RPE-1 cells to activate PPG-idasanutlin. Laser target area (represented by red circle) for single pulse (0.1 s irradiation at 5 μm interspaced position) indicated with scale. Individual irradiated cells followed by measuring nuclear p53-venus levels (fluorescence) every 15 min for 3 h after laser irradiation. Approximately 200 cells in each microwell. (b) Percentage of cells that divide within 8 h after indicated treatments.38 (c,d) p53-venus fluorescent signal in individual RPE-1 cells tracked over time after indicated treatments as represented in (a).39