Fig. 3.

The number of bacterial cells of all the tested D. zeae strains invading into potato (a), cabbage (b), banana (c) and clivia (d), respectively. Healthy plant materials were surface-sterilized and inoculated with 2 μL of bacterial overnight cultures (OD₆₀₀ = 2.0) in LB medium, and incubated at 28 °C. Tissues were taken out after 12 h and 24 h, respectively. And the diseased and surrounding healthy tissues in equal weight were cut and ground, and then added with 10 mL of sterilized 0.85% NaCl solution, stirred evenly, and 1 mL of which was diluted in series gradients, and 100 μL in each gradient was spread evenly onto LB agar plates in triplicates and kept at 28 °C for 24 h. Colonies between 30 to 300 CFU were counted. LB medium was used as a negative control. Each assay was repeated three times with duplicates