Fig. 2.

Imaging of cytotoxicity of Sudanese medicinal plants in HeLa cells using the HCS DNA Damage Kit. AN: Acacia nilotica, ZS: Ziziphus spina-christi, GA: Geigeria alata. MA: Martynia annua AP: Abrus precatorius; For each extract, 70 represents 70% EtOH extract and W represents water extract. As a control experiment, HeLa cells were treated with 120 μM valinomycin for 24 h at 37 °C/5% CO₂. Imaging and analysis were performed using a BZ-X700. At 120 μM valinomycin, cells showed cytotoxic effects as demonstrated by the positive Image-iT® DEAD Green™ viability stain fluorescence