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. 2018 Oct 19;18:138. doi: 10.1186/s12866-018-1307-4

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Duck endothelial cells can be isolated from the aorta of embryonated eggs. Representative immunofluorescence images and FACS plots of duck aortic endothelial cells (a) and chicken aortic endothelial cells (b) following a 4 hour incubation with Alexa Flour®488 conjugated Ac-LDL. Duck and chicken aortic endothelial cells were passaged 15 times and 17 times respectively in EGM^TM-2MV medium. EA-hy926 and NCl-H441 cells were used as positive and negative control respectively for the uptake of Ac-LDL. Scale bar: 100 nm